rabbit polyclonal anti phospho smad1 Search Results


rabbit anti psmad1 5 8  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit anti psmad1 5 8
Rabbit Anti Psmad1 5 8, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti smad1  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc rabbit anti smad1
a Scheme of cell surface competitive binding assays in ( b – e ). b Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. c Quantification of ( b ). d Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. e Quantification of ( d ). f Amino acid sequence comparison of TK-KC peptides in RSPO2 TSP1 domain of several species. Note that TK-KC peptide sequence is highly conserved (Magenta boxes). g Amino acid sequence comparison of RSPO1-4 TSP1 domains. Note that TK-KC peptide sequence derived from human RSPO2 TSP1 domain is unconserved in other RSPOs (Magenta boxes). h Microinjection strategy for ( i–k ). i Western blot analysis of phosphorylated ERK1/2 and <t>Smad1</t> (pERK1/2 and pSmad1) and total ERK1/2 and Smad1 (tERK1/2 and tSmad1) in Xenopus embryo (St. 15) lysates. j WISH of dand5 in Xenopus LRO (St. 19) injected as indicated. Arrowheads, dand5 derepression. Scale bar, 100 µm. k Quantification of ( j ). Two-sided Fisher’s exact test used for statistical analysis. n = number of dorso-posterior explants. l Model showing the mode of action for TK to intervene RSPO2-FGFR4 interaction and increases FGFR4 signaling. Data information: For all cell surface binding assays ( c , e ), data are displayed as mean ± SD with two-tailed unpaired t -test: n = 3 biologically independent samples. Source data are provided as a Source Data file.
Rabbit Anti Smad1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti smad1/product/Cell Signaling Technology Inc
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rabbit anti smad1 - by Bioz Stars, 2026-02
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anti p smad1 5 9  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc anti p smad1 5 9
a Scheme of cell surface competitive binding assays in ( b – e ). b Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. c Quantification of ( b ). d Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. e Quantification of ( d ). f Amino acid sequence comparison of TK-KC peptides in RSPO2 TSP1 domain of several species. Note that TK-KC peptide sequence is highly conserved (Magenta boxes). g Amino acid sequence comparison of RSPO1-4 TSP1 domains. Note that TK-KC peptide sequence derived from human RSPO2 TSP1 domain is unconserved in other RSPOs (Magenta boxes). h Microinjection strategy for ( i–k ). i Western blot analysis of phosphorylated ERK1/2 and <t>Smad1</t> (pERK1/2 and pSmad1) and total ERK1/2 and Smad1 (tERK1/2 and tSmad1) in Xenopus embryo (St. 15) lysates. j WISH of dand5 in Xenopus LRO (St. 19) injected as indicated. Arrowheads, dand5 derepression. Scale bar, 100 µm. k Quantification of ( j ). Two-sided Fisher’s exact test used for statistical analysis. n = number of dorso-posterior explants. l Model showing the mode of action for TK to intervene RSPO2-FGFR4 interaction and increases FGFR4 signaling. Data information: For all cell surface binding assays ( c , e ), data are displayed as mean ± SD with two-tailed unpaired t -test: n = 3 biologically independent samples. Source data are provided as a Source Data file.
Anti P Smad1 5 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyclonal rabbit anti phospho smad5  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc polyclonal rabbit anti phospho smad5
a Scheme of cell surface competitive binding assays in ( b – e ). b Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. c Quantification of ( b ). d Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. e Quantification of ( d ). f Amino acid sequence comparison of TK-KC peptides in RSPO2 TSP1 domain of several species. Note that TK-KC peptide sequence is highly conserved (Magenta boxes). g Amino acid sequence comparison of RSPO1-4 TSP1 domains. Note that TK-KC peptide sequence derived from human RSPO2 TSP1 domain is unconserved in other RSPOs (Magenta boxes). h Microinjection strategy for ( i–k ). i Western blot analysis of phosphorylated ERK1/2 and <t>Smad1</t> (pERK1/2 and pSmad1) and total ERK1/2 and Smad1 (tERK1/2 and tSmad1) in Xenopus embryo (St. 15) lysates. j WISH of dand5 in Xenopus LRO (St. 19) injected as indicated. Arrowheads, dand5 derepression. Scale bar, 100 µm. k Quantification of ( j ). Two-sided Fisher’s exact test used for statistical analysis. n = number of dorso-posterior explants. l Model showing the mode of action for TK to intervene RSPO2-FGFR4 interaction and increases FGFR4 signaling. Data information: For all cell surface binding assays ( c , e ), data are displayed as mean ± SD with two-tailed unpaired t -test: n = 3 biologically independent samples. Source data are provided as a Source Data file.
Polyclonal Rabbit Anti Phospho Smad5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti p smad1 5 8 antibody  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc rabbit anti p smad1 5 8 antibody
a Scheme of cell surface competitive binding assays in ( b – e ). b Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. c Quantification of ( b ). d Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. e Quantification of ( d ). f Amino acid sequence comparison of TK-KC peptides in RSPO2 TSP1 domain of several species. Note that TK-KC peptide sequence is highly conserved (Magenta boxes). g Amino acid sequence comparison of RSPO1-4 TSP1 domains. Note that TK-KC peptide sequence derived from human RSPO2 TSP1 domain is unconserved in other RSPOs (Magenta boxes). h Microinjection strategy for ( i–k ). i Western blot analysis of phosphorylated ERK1/2 and <t>Smad1</t> (pERK1/2 and pSmad1) and total ERK1/2 and Smad1 (tERK1/2 and tSmad1) in Xenopus embryo (St. 15) lysates. j WISH of dand5 in Xenopus LRO (St. 19) injected as indicated. Arrowheads, dand5 derepression. Scale bar, 100 µm. k Quantification of ( j ). Two-sided Fisher’s exact test used for statistical analysis. n = number of dorso-posterior explants. l Model showing the mode of action for TK to intervene RSPO2-FGFR4 interaction and increases FGFR4 signaling. Data information: For all cell surface binding assays ( c , e ), data are displayed as mean ± SD with two-tailed unpaired t -test: n = 3 biologically independent samples. Source data are provided as a Source Data file.
Rabbit Anti P Smad1 5 8 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti p smad1 5 8 antibody - by Bioz Stars, 2026-02
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anti smad 1  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti smad 1
a Scheme of cell surface competitive binding assays in ( b – e ). b Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. c Quantification of ( b ). d Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. e Quantification of ( d ). f Amino acid sequence comparison of TK-KC peptides in RSPO2 TSP1 domain of several species. Note that TK-KC peptide sequence is highly conserved (Magenta boxes). g Amino acid sequence comparison of RSPO1-4 TSP1 domains. Note that TK-KC peptide sequence derived from human RSPO2 TSP1 domain is unconserved in other RSPOs (Magenta boxes). h Microinjection strategy for ( i–k ). i Western blot analysis of phosphorylated ERK1/2 and <t>Smad1</t> (pERK1/2 and pSmad1) and total ERK1/2 and Smad1 (tERK1/2 and tSmad1) in Xenopus embryo (St. 15) lysates. j WISH of dand5 in Xenopus LRO (St. 19) injected as indicated. Arrowheads, dand5 derepression. Scale bar, 100 µm. k Quantification of ( j ). Two-sided Fisher’s exact test used for statistical analysis. n = number of dorso-posterior explants. l Model showing the mode of action for TK to intervene RSPO2-FGFR4 interaction and increases FGFR4 signaling. Data information: For all cell surface binding assays ( c , e ), data are displayed as mean ± SD with two-tailed unpaired t -test: n = 3 biologically independent samples. Source data are provided as a Source Data file.
Anti Smad 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti smad 1/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
anti smad 1 - by Bioz Stars, 2026-02
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anti p smad1 5 8  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc anti p smad1 5 8
a Scheme of cell surface competitive binding assays in ( b – e ). b Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. c Quantification of ( b ). d Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. e Quantification of ( d ). f Amino acid sequence comparison of TK-KC peptides in RSPO2 TSP1 domain of several species. Note that TK-KC peptide sequence is highly conserved (Magenta boxes). g Amino acid sequence comparison of RSPO1-4 TSP1 domains. Note that TK-KC peptide sequence derived from human RSPO2 TSP1 domain is unconserved in other RSPOs (Magenta boxes). h Microinjection strategy for ( i–k ). i Western blot analysis of phosphorylated ERK1/2 and <t>Smad1</t> (pERK1/2 and pSmad1) and total ERK1/2 and Smad1 (tERK1/2 and tSmad1) in Xenopus embryo (St. 15) lysates. j WISH of dand5 in Xenopus LRO (St. 19) injected as indicated. Arrowheads, dand5 derepression. Scale bar, 100 µm. k Quantification of ( j ). Two-sided Fisher’s exact test used for statistical analysis. n = number of dorso-posterior explants. l Model showing the mode of action for TK to intervene RSPO2-FGFR4 interaction and increases FGFR4 signaling. Data information: For all cell surface binding assays ( c , e ), data are displayed as mean ± SD with two-tailed unpaired t -test: n = 3 biologically independent samples. Source data are provided as a Source Data file.
Anti P Smad1 5 8, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p smad1 5 8/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
anti p smad1 5 8 - by Bioz Stars, 2026-02
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rabbit anti phospho smad1 5 8  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc rabbit anti phospho smad1 5 8
a Scheme of cell surface competitive binding assays in ( b – e ). b Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. c Quantification of ( b ). d Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. e Quantification of ( d ). f Amino acid sequence comparison of TK-KC peptides in RSPO2 TSP1 domain of several species. Note that TK-KC peptide sequence is highly conserved (Magenta boxes). g Amino acid sequence comparison of RSPO1-4 TSP1 domains. Note that TK-KC peptide sequence derived from human RSPO2 TSP1 domain is unconserved in other RSPOs (Magenta boxes). h Microinjection strategy for ( i–k ). i Western blot analysis of phosphorylated ERK1/2 and <t>Smad1</t> (pERK1/2 and pSmad1) and total ERK1/2 and Smad1 (tERK1/2 and tSmad1) in Xenopus embryo (St. 15) lysates. j WISH of dand5 in Xenopus LRO (St. 19) injected as indicated. Arrowheads, dand5 derepression. Scale bar, 100 µm. k Quantification of ( j ). Two-sided Fisher’s exact test used for statistical analysis. n = number of dorso-posterior explants. l Model showing the mode of action for TK to intervene RSPO2-FGFR4 interaction and increases FGFR4 signaling. Data information: For all cell surface binding assays ( c , e ), data are displayed as mean ± SD with two-tailed unpaired t -test: n = 3 biologically independent samples. Source data are provided as a Source Data file.
Rabbit Anti Phospho Smad1 5 8, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti phospho smad1 5 8/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
rabbit anti phospho smad1 5 8 - by Bioz Stars, 2026-02
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rabbit polyclonal anti-smad1 antibody  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc rabbit polyclonal anti-smad1 antibody
a Scheme of cell surface competitive binding assays in ( b – e ). b Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. c Quantification of ( b ). d Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. e Quantification of ( d ). f Amino acid sequence comparison of TK-KC peptides in RSPO2 TSP1 domain of several species. Note that TK-KC peptide sequence is highly conserved (Magenta boxes). g Amino acid sequence comparison of RSPO1-4 TSP1 domains. Note that TK-KC peptide sequence derived from human RSPO2 TSP1 domain is unconserved in other RSPOs (Magenta boxes). h Microinjection strategy for ( i–k ). i Western blot analysis of phosphorylated ERK1/2 and <t>Smad1</t> (pERK1/2 and pSmad1) and total ERK1/2 and Smad1 (tERK1/2 and tSmad1) in Xenopus embryo (St. 15) lysates. j WISH of dand5 in Xenopus LRO (St. 19) injected as indicated. Arrowheads, dand5 derepression. Scale bar, 100 µm. k Quantification of ( j ). Two-sided Fisher’s exact test used for statistical analysis. n = number of dorso-posterior explants. l Model showing the mode of action for TK to intervene RSPO2-FGFR4 interaction and increases FGFR4 signaling. Data information: For all cell surface binding assays ( c , e ), data are displayed as mean ± SD with two-tailed unpaired t -test: n = 3 biologically independent samples. Source data are provided as a Source Data file.
Rabbit Polyclonal Anti Smad1 Antibody, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal anti-smad1 antibody - by Bioz Stars, 2026-02
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rabbit polyclonal anti smad1  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology rabbit polyclonal anti smad1
a Scheme of cell surface competitive binding assays in ( b – e ). b Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. c Quantification of ( b ). d Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. e Quantification of ( d ). f Amino acid sequence comparison of TK-KC peptides in RSPO2 TSP1 domain of several species. Note that TK-KC peptide sequence is highly conserved (Magenta boxes). g Amino acid sequence comparison of RSPO1-4 TSP1 domains. Note that TK-KC peptide sequence derived from human RSPO2 TSP1 domain is unconserved in other RSPOs (Magenta boxes). h Microinjection strategy for ( i–k ). i Western blot analysis of phosphorylated ERK1/2 and <t>Smad1</t> (pERK1/2 and pSmad1) and total ERK1/2 and Smad1 (tERK1/2 and tSmad1) in Xenopus embryo (St. 15) lysates. j WISH of dand5 in Xenopus LRO (St. 19) injected as indicated. Arrowheads, dand5 derepression. Scale bar, 100 µm. k Quantification of ( j ). Two-sided Fisher’s exact test used for statistical analysis. n = number of dorso-posterior explants. l Model showing the mode of action for TK to intervene RSPO2-FGFR4 interaction and increases FGFR4 signaling. Data information: For all cell surface binding assays ( c , e ), data are displayed as mean ± SD with two-tailed unpaired t -test: n = 3 biologically independent samples. Source data are provided as a Source Data file.
Rabbit Polyclonal Anti Smad1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti smad1 2 3 mab h 2  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology anti smad1 2 3 mab h 2
a Scheme of cell surface competitive binding assays in ( b – e ). b Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. c Quantification of ( b ). d Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. e Quantification of ( d ). f Amino acid sequence comparison of TK-KC peptides in RSPO2 TSP1 domain of several species. Note that TK-KC peptide sequence is highly conserved (Magenta boxes). g Amino acid sequence comparison of RSPO1-4 TSP1 domains. Note that TK-KC peptide sequence derived from human RSPO2 TSP1 domain is unconserved in other RSPOs (Magenta boxes). h Microinjection strategy for ( i–k ). i Western blot analysis of phosphorylated ERK1/2 and <t>Smad1</t> (pERK1/2 and pSmad1) and total ERK1/2 and Smad1 (tERK1/2 and tSmad1) in Xenopus embryo (St. 15) lysates. j WISH of dand5 in Xenopus LRO (St. 19) injected as indicated. Arrowheads, dand5 derepression. Scale bar, 100 µm. k Quantification of ( j ). Two-sided Fisher’s exact test used for statistical analysis. n = number of dorso-posterior explants. l Model showing the mode of action for TK to intervene RSPO2-FGFR4 interaction and increases FGFR4 signaling. Data information: For all cell surface binding assays ( c , e ), data are displayed as mean ± SD with two-tailed unpaired t -test: n = 3 biologically independent samples. Source data are provided as a Source Data file.
Anti Smad1 2 3 Mab H 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyclonal rabbit anti-human psmad1/5/8 antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc polyclonal rabbit anti-human psmad1/5/8 antibody
a Scheme of cell surface competitive binding assays in ( b – e ). b Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. c Quantification of ( b ). d Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. e Quantification of ( d ). f Amino acid sequence comparison of TK-KC peptides in RSPO2 TSP1 domain of several species. Note that TK-KC peptide sequence is highly conserved (Magenta boxes). g Amino acid sequence comparison of RSPO1-4 TSP1 domains. Note that TK-KC peptide sequence derived from human RSPO2 TSP1 domain is unconserved in other RSPOs (Magenta boxes). h Microinjection strategy for ( i–k ). i Western blot analysis of phosphorylated ERK1/2 and <t>Smad1</t> (pERK1/2 and pSmad1) and total ERK1/2 and Smad1 (tERK1/2 and tSmad1) in Xenopus embryo (St. 15) lysates. j WISH of dand5 in Xenopus LRO (St. 19) injected as indicated. Arrowheads, dand5 derepression. Scale bar, 100 µm. k Quantification of ( j ). Two-sided Fisher’s exact test used for statistical analysis. n = number of dorso-posterior explants. l Model showing the mode of action for TK to intervene RSPO2-FGFR4 interaction and increases FGFR4 signaling. Data information: For all cell surface binding assays ( c , e ), data are displayed as mean ± SD with two-tailed unpaired t -test: n = 3 biologically independent samples. Source data are provided as a Source Data file.
Polyclonal Rabbit Anti Human Psmad1/5/8 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Image Search Results


a Scheme of cell surface competitive binding assays in ( b – e ). b Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. c Quantification of ( b ). d Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. e Quantification of ( d ). f Amino acid sequence comparison of TK-KC peptides in RSPO2 TSP1 domain of several species. Note that TK-KC peptide sequence is highly conserved (Magenta boxes). g Amino acid sequence comparison of RSPO1-4 TSP1 domains. Note that TK-KC peptide sequence derived from human RSPO2 TSP1 domain is unconserved in other RSPOs (Magenta boxes). h Microinjection strategy for ( i–k ). i Western blot analysis of phosphorylated ERK1/2 and Smad1 (pERK1/2 and pSmad1) and total ERK1/2 and Smad1 (tERK1/2 and tSmad1) in Xenopus embryo (St. 15) lysates. j WISH of dand5 in Xenopus LRO (St. 19) injected as indicated. Arrowheads, dand5 derepression. Scale bar, 100 µm. k Quantification of ( j ). Two-sided Fisher’s exact test used for statistical analysis. n = number of dorso-posterior explants. l Model showing the mode of action for TK to intervene RSPO2-FGFR4 interaction and increases FGFR4 signaling. Data information: For all cell surface binding assays ( c , e ), data are displayed as mean ± SD with two-tailed unpaired t -test: n = 3 biologically independent samples. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: R-Spondin 2 governs Xenopus left-right body axis formation by establishing an FGF signaling gradient

doi: 10.1038/s41467-024-44951-7

Figure Lengend Snippet: a Scheme of cell surface competitive binding assays in ( b – e ). b Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. c Quantification of ( b ). d Representative images of HEK293T cells transfected and treated as indicated. Scale bar, 1 mm. e Quantification of ( d ). f Amino acid sequence comparison of TK-KC peptides in RSPO2 TSP1 domain of several species. Note that TK-KC peptide sequence is highly conserved (Magenta boxes). g Amino acid sequence comparison of RSPO1-4 TSP1 domains. Note that TK-KC peptide sequence derived from human RSPO2 TSP1 domain is unconserved in other RSPOs (Magenta boxes). h Microinjection strategy for ( i–k ). i Western blot analysis of phosphorylated ERK1/2 and Smad1 (pERK1/2 and pSmad1) and total ERK1/2 and Smad1 (tERK1/2 and tSmad1) in Xenopus embryo (St. 15) lysates. j WISH of dand5 in Xenopus LRO (St. 19) injected as indicated. Arrowheads, dand5 derepression. Scale bar, 100 µm. k Quantification of ( j ). Two-sided Fisher’s exact test used for statistical analysis. n = number of dorso-posterior explants. l Model showing the mode of action for TK to intervene RSPO2-FGFR4 interaction and increases FGFR4 signaling. Data information: For all cell surface binding assays ( c , e ), data are displayed as mean ± SD with two-tailed unpaired t -test: n = 3 biologically independent samples. Source data are provided as a Source Data file.

Article Snippet: Primary antibodies used for western blotting: Rabbit anti-Phospho-ERK1/2 (Cell Signaling Technology 9101 S); Rabbit anti-ERK1/2 (Sigma M5670); Mouse anti-beta-Catenin (BD 610154); Rabbit anti-LRP6 (Cell Signaling Technology 2560); Rabbit anti-GAPDH (Cell Signaling Technology 2118 S); Goat anti-RSPO2 (R and D systems AF3266); Rat anti-HA (Roche 11867423001); Rabbit anti-Phospho-Smad1 (Cell Signaling Technology 9516); Rabbit anti-Smad1(Cell Signaling Technology 9743 S).

Techniques: Binding Assay, Transfection, Sequencing, Comparison, Derivative Assay, Western Blot, Injection, Two Tailed Test